anti caspase 3 antibody Search Results


caspase 3  (Bioss)
95
Bioss caspase 3
Western plot ( A ) and immunohistochemistry ( E–F ) of myocardial <t>caspase</t> <t>3,</t> and PARP and apoptosis TUNEL stain in IR myocardium. The statistic data in <t>Caspase</t> <t>3</t> ( B ), PARP ( C ) and TUNEL positive nuclei ( D ) are indicated. Data are expressed as mean ± SEM ( n = 3) using the single values. * p < 0.05 vs. CON. a p < 0.05 vs. STZ. b p < 0.05 vs. IR CON.
Caspase 3, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cl caspase 3  (StressMarq)
90
StressMarq cl caspase 3
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Cl Caspase 3, supplied by StressMarq, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cl caspase 3 - by Bioz Stars, 2026-02
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caspase 3  (Boster Bio)
96
Boster Bio caspase 3
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Caspase 3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 3/product/Boster Bio
Average 96 stars, based on 1 article reviews
caspase 3 - by Bioz Stars, 2026-02
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ab32356 rrid ab 732924  (Abcam)
96
Abcam ab32356 rrid ab 732924
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Ab32356 Rrid Ab 732924, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody anti cleavedcaspase 3  (St Johns Laboratory)
93
St Johns Laboratory antibody anti cleavedcaspase 3
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Antibody Anti Cleavedcaspase 3, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti cleavedcaspase 3/product/St Johns Laboratory
Average 93 stars, based on 1 article reviews
antibody anti cleavedcaspase 3 - by Bioz Stars, 2026-02
93/100 stars
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bio, cat. no  (Boster Bio)
93
Boster Bio bio, cat. no
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Bio, Cat. No, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
bio, cat. no - by Bioz Stars, 2026-02
93/100 stars
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mouse anti caspase3  (Boster Bio)
90
Boster Bio mouse anti caspase3
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Mouse Anti Caspase3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti caspase3/product/Boster Bio
Average 90 stars, based on 1 article reviews
mouse anti caspase3 - by Bioz Stars, 2026-02
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rabbit anti caspase 3 monoclonal antibody  (Boster Bio)
92
Boster Bio rabbit anti caspase 3 monoclonal antibody
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Rabbit Anti Caspase 3 Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
rabbit anti caspase 3 monoclonal antibody - by Bioz Stars, 2026-02
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caspase 3 p12 antibody  (Boster Bio)
93
Boster Bio caspase 3 p12 antibody
IHC assay of the intestinal cancer colo320 xenograft sample from nude mice treated by BF-rTK/GCV ( n = 3). ( A ) The tumor tissue samples were from intestinal cancer colo320 cells treated by PBS/GCV, and BF-rTK/GCV, respectively. The caspase 8 and <t>caspase</t> <t>3</t> levels were analyzed with IHC; ( B ) Quantitative analysis of caspase 8 and <t>caspase</t> <t>3</t> (* p < 0.05).
Caspase 3 P12 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase 3 p12 antibody - by Bioz Stars, 2026-02
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anti caspase 3  (St Johns Laboratory)
91
St Johns Laboratory anti caspase 3
IHC assay of the intestinal cancer colo320 xenograft sample from nude mice treated by BF-rTK/GCV ( n = 3). ( A ) The tumor tissue samples were from intestinal cancer colo320 cells treated by PBS/GCV, and BF-rTK/GCV, respectively. The caspase 8 and <t>caspase</t> <t>3</t> levels were analyzed with IHC; ( B ) Quantitative analysis of caspase 8 and <t>caspase</t> <t>3</t> (* p < 0.05).
Anti Caspase 3, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti caspase 3/product/St Johns Laboratory
Average 91 stars, based on 1 article reviews
anti caspase 3 - by Bioz Stars, 2026-02
91/100 stars
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rabbit polyclonal caspase 3 antibody  (Boster Bio)
91
Boster Bio rabbit polyclonal caspase 3 antibody
IHC assay of the intestinal cancer colo320 xenograft sample from nude mice treated by BF-rTK/GCV ( n = 3). ( A ) The tumor tissue samples were from intestinal cancer colo320 cells treated by PBS/GCV, and BF-rTK/GCV, respectively. The caspase 8 and <t>caspase</t> <t>3</t> levels were analyzed with IHC; ( B ) Quantitative analysis of caspase 8 and <t>caspase</t> <t>3</t> (* p < 0.05).
Rabbit Polyclonal Caspase 3 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal caspase 3 antibody/product/Boster Bio
Average 91 stars, based on 1 article reviews
rabbit polyclonal caspase 3 antibody - by Bioz Stars, 2026-02
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Image Search Results


Western plot ( A ) and immunohistochemistry ( E–F ) of myocardial caspase 3, and PARP and apoptosis TUNEL stain in IR myocardium. The statistic data in Caspase 3 ( B ), PARP ( C ) and TUNEL positive nuclei ( D ) are indicated. Data are expressed as mean ± SEM ( n = 3) using the single values. * p < 0.05 vs. CON. a p < 0.05 vs. STZ. b p < 0.05 vs. IR CON.

Journal: Antioxidants

Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

doi: 10.3390/antiox9080679

Figure Lengend Snippet: Western plot ( A ) and immunohistochemistry ( E–F ) of myocardial caspase 3, and PARP and apoptosis TUNEL stain in IR myocardium. The statistic data in Caspase 3 ( B ), PARP ( C ) and TUNEL positive nuclei ( D ) are indicated. Data are expressed as mean ± SEM ( n = 3) using the single values. * p < 0.05 vs. CON. a p < 0.05 vs. STZ. b p < 0.05 vs. IR CON.

Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

Techniques: Western Blot, Immunohistochemistry, TUNEL Assay, Staining

ZQT selectively induced G-MDSC apoptosis by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: Mediators of Inflammation

Article Title: Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer

doi: 10.1155/2021/8856326

Figure Lengend Snippet: ZQT selectively induced G-MDSC apoptosis by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The following reagents were used: Matrigel basement membrane matrix (Corning, CAT#356234); Hygromycin B (MK Bio, CAT#MS0003-1G); FBS (fetal bovine serum), PS (penicillin-streptomycin), DMEM (Dulbecco's modified Eagle's medium), Trypsin-EDTA (0.25%) (Thermo Fisher Scientific, CAT#10091148, 15140122, 11995065, and 25200072); XenoLight D-Luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, CAT#122799); Brilliant Violet 510™ anti-mouse CD335 (NKp46), PE/Dazzle™ 594 anti-mouse CD11c, Brilliant Violet 650™ anti-mouse I-A/I-E, Brilliant Violet 421™ anti-mouse F4/80, Alexa Fluor® 700 anti-mouse CD4, Brilliant Violet 605™ anti-mouse CD8a, APC anti-mouse CD8, FITC anti-mouse CD4, PE anti-mouse CD3, FITC anti-mouse CD11b, PE anti-mouse Gr1, APC anti-mouse Ly6G, PerCP5.5 anti-mouse Ly6C, purified anti-mouse CD28, PE-Cy7 anti-mouse CD107 α (LAMP-1) (BioLegend, CAT#137623, 117347, 107641, 123137, 100536, 100743, 100712, 100406, 100205, 101206, 108407, 127614, 128011, 102101, and 121619); Red Cell Lysis (Biosharp, CAT#BL503B); CFSE (Sigma-Aldrich, CAT#21888); EasySep Mouse T Cell Isolation Kit, EasySep Mouse MDSC Isolation Kit (STEMCELL, CAT#19851 and 19867); InVivoMab anti-mouse Ly6G (1A8), InVivoMab rat IgG2a isotype control (Bio X Cell, CAT#BE0075-1 and BE0089); Mouse IFN- γ ELISA Set (BD Biosciences, CAT#555138); STAT1, p-STAT1, STAT3, p-STAT3, S100A9, cl-PARP, Arg-1, Ly6G, Cytochrome c, Cyclin D1 (Cell Signaling Technology, CAT#14994, 58D6, 9139T, 4113S, 73425, 9548S, 93668, 87048, 11940T, and 55506T); 1x TBS (Tris-buffered saline) buffer, Tween 20 (Sangon Biotech, CAT#C508113 and A600560); Immobilon Western Chemiluminescent HRP Substrate (Millipore, CAT# WBKLS0500); rabbit anti-Ki67 antibody (Abcam, CAT#ab16667); anti-Bcl-2 (rabbit) antibody (Rockland Immunochemicals, CAT# 200-401-Z43); purified anti-Bax (Biolegend, CAT#633601); cl-caspase-3 (StressMarq Biosciences Inc., CAT# SPC-1319); DeadEnd Fluorometric TUNEL System (Promega, CAT#REF: G3250); Reactive Oxygen Species Assay Kit (Beyotime, CAT#S0033S), Cell Cycle and Apoptosis Analysis Kit (Beyotime, CAT#S0033S and C1052).

Techniques: Isolation, Expressing, Western Blot, Staining, Lymphocyte Proliferation Assay, Incubation, Cell Stimulation, Flow Cytometry

ZQT induced apoptosis in G-MDSCs in TME and inhibited its immunosuppressive activity by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. Mice were treated with anti-Ly6G neutralizing antibody or isotype control by intraperitoneal injection for 14 days before surgery, after which model mice were given maintenance treatment every other day. (a, b) The bioluminescence images of model mice on day 7 and day 28 after surgery. (c) Survival curves. (d, e) The percentage of G-MDSCs, CD3 + T cells, CD4 + T cells, and CD8 + T cells in tumor tissue were analyzed by flow cytometry. (f, g) CD3 + T cells isolated from the tumor tissue of tumor-bearing C57BL/6 mice in different groups were cocultured with LLC cells for 4 h before they were incubated with CD3, CD8, and CD107 α antibodies. Events shown were finally gated on CD3 and CD8. (h) ELISA analysis for the expression of IFN- γ in cells from tumor tissue. (i) RT-qPCR analysis of Arg-1, iNOS, and S100A9 gene expression and flow cytometry for ROS production. (j) IHC staining for Ly6G and CD8 in tumor tissue (scale bar: 50 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: Mediators of Inflammation

Article Title: Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer

doi: 10.1155/2021/8856326

Figure Lengend Snippet: ZQT induced apoptosis in G-MDSCs in TME and inhibited its immunosuppressive activity by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. Mice were treated with anti-Ly6G neutralizing antibody or isotype control by intraperitoneal injection for 14 days before surgery, after which model mice were given maintenance treatment every other day. (a, b) The bioluminescence images of model mice on day 7 and day 28 after surgery. (c) Survival curves. (d, e) The percentage of G-MDSCs, CD3 + T cells, CD4 + T cells, and CD8 + T cells in tumor tissue were analyzed by flow cytometry. (f, g) CD3 + T cells isolated from the tumor tissue of tumor-bearing C57BL/6 mice in different groups were cocultured with LLC cells for 4 h before they were incubated with CD3, CD8, and CD107 α antibodies. Events shown were finally gated on CD3 and CD8. (h) ELISA analysis for the expression of IFN- γ in cells from tumor tissue. (i) RT-qPCR analysis of Arg-1, iNOS, and S100A9 gene expression and flow cytometry for ROS production. (j) IHC staining for Ly6G and CD8 in tumor tissue (scale bar: 50 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The following reagents were used: Matrigel basement membrane matrix (Corning, CAT#356234); Hygromycin B (MK Bio, CAT#MS0003-1G); FBS (fetal bovine serum), PS (penicillin-streptomycin), DMEM (Dulbecco's modified Eagle's medium), Trypsin-EDTA (0.25%) (Thermo Fisher Scientific, CAT#10091148, 15140122, 11995065, and 25200072); XenoLight D-Luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, CAT#122799); Brilliant Violet 510™ anti-mouse CD335 (NKp46), PE/Dazzle™ 594 anti-mouse CD11c, Brilliant Violet 650™ anti-mouse I-A/I-E, Brilliant Violet 421™ anti-mouse F4/80, Alexa Fluor® 700 anti-mouse CD4, Brilliant Violet 605™ anti-mouse CD8a, APC anti-mouse CD8, FITC anti-mouse CD4, PE anti-mouse CD3, FITC anti-mouse CD11b, PE anti-mouse Gr1, APC anti-mouse Ly6G, PerCP5.5 anti-mouse Ly6C, purified anti-mouse CD28, PE-Cy7 anti-mouse CD107 α (LAMP-1) (BioLegend, CAT#137623, 117347, 107641, 123137, 100536, 100743, 100712, 100406, 100205, 101206, 108407, 127614, 128011, 102101, and 121619); Red Cell Lysis (Biosharp, CAT#BL503B); CFSE (Sigma-Aldrich, CAT#21888); EasySep Mouse T Cell Isolation Kit, EasySep Mouse MDSC Isolation Kit (STEMCELL, CAT#19851 and 19867); InVivoMab anti-mouse Ly6G (1A8), InVivoMab rat IgG2a isotype control (Bio X Cell, CAT#BE0075-1 and BE0089); Mouse IFN- γ ELISA Set (BD Biosciences, CAT#555138); STAT1, p-STAT1, STAT3, p-STAT3, S100A9, cl-PARP, Arg-1, Ly6G, Cytochrome c, Cyclin D1 (Cell Signaling Technology, CAT#14994, 58D6, 9139T, 4113S, 73425, 9548S, 93668, 87048, 11940T, and 55506T); 1x TBS (Tris-buffered saline) buffer, Tween 20 (Sangon Biotech, CAT#C508113 and A600560); Immobilon Western Chemiluminescent HRP Substrate (Millipore, CAT# WBKLS0500); rabbit anti-Ki67 antibody (Abcam, CAT#ab16667); anti-Bcl-2 (rabbit) antibody (Rockland Immunochemicals, CAT# 200-401-Z43); purified anti-Bax (Biolegend, CAT#633601); cl-caspase-3 (StressMarq Biosciences Inc., CAT# SPC-1319); DeadEnd Fluorometric TUNEL System (Promega, CAT#REF: G3250); Reactive Oxygen Species Assay Kit (Beyotime, CAT#S0033S), Cell Cycle and Apoptosis Analysis Kit (Beyotime, CAT#S0033S and C1052).

Techniques: Activity Assay, Injection, Flow Cytometry, Isolation, Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Immunohistochemistry

The immune regulatory activity of TCM formula ZQT in TME. ZQT induces the apoptosis of G-MDSCs via the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway, resulting in a significant decrease in G-MDSCs and their immunosuppressive activity, consequently promoting the infiltration and killing activity of CD8 + T cells, leading to the inhibition of the proliferation of tumor cells.

Journal: Mediators of Inflammation

Article Title: Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer

doi: 10.1155/2021/8856326

Figure Lengend Snippet: The immune regulatory activity of TCM formula ZQT in TME. ZQT induces the apoptosis of G-MDSCs via the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway, resulting in a significant decrease in G-MDSCs and their immunosuppressive activity, consequently promoting the infiltration and killing activity of CD8 + T cells, leading to the inhibition of the proliferation of tumor cells.

Article Snippet: The following reagents were used: Matrigel basement membrane matrix (Corning, CAT#356234); Hygromycin B (MK Bio, CAT#MS0003-1G); FBS (fetal bovine serum), PS (penicillin-streptomycin), DMEM (Dulbecco's modified Eagle's medium), Trypsin-EDTA (0.25%) (Thermo Fisher Scientific, CAT#10091148, 15140122, 11995065, and 25200072); XenoLight D-Luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, CAT#122799); Brilliant Violet 510™ anti-mouse CD335 (NKp46), PE/Dazzle™ 594 anti-mouse CD11c, Brilliant Violet 650™ anti-mouse I-A/I-E, Brilliant Violet 421™ anti-mouse F4/80, Alexa Fluor® 700 anti-mouse CD4, Brilliant Violet 605™ anti-mouse CD8a, APC anti-mouse CD8, FITC anti-mouse CD4, PE anti-mouse CD3, FITC anti-mouse CD11b, PE anti-mouse Gr1, APC anti-mouse Ly6G, PerCP5.5 anti-mouse Ly6C, purified anti-mouse CD28, PE-Cy7 anti-mouse CD107 α (LAMP-1) (BioLegend, CAT#137623, 117347, 107641, 123137, 100536, 100743, 100712, 100406, 100205, 101206, 108407, 127614, 128011, 102101, and 121619); Red Cell Lysis (Biosharp, CAT#BL503B); CFSE (Sigma-Aldrich, CAT#21888); EasySep Mouse T Cell Isolation Kit, EasySep Mouse MDSC Isolation Kit (STEMCELL, CAT#19851 and 19867); InVivoMab anti-mouse Ly6G (1A8), InVivoMab rat IgG2a isotype control (Bio X Cell, CAT#BE0075-1 and BE0089); Mouse IFN- γ ELISA Set (BD Biosciences, CAT#555138); STAT1, p-STAT1, STAT3, p-STAT3, S100A9, cl-PARP, Arg-1, Ly6G, Cytochrome c, Cyclin D1 (Cell Signaling Technology, CAT#14994, 58D6, 9139T, 4113S, 73425, 9548S, 93668, 87048, 11940T, and 55506T); 1x TBS (Tris-buffered saline) buffer, Tween 20 (Sangon Biotech, CAT#C508113 and A600560); Immobilon Western Chemiluminescent HRP Substrate (Millipore, CAT# WBKLS0500); rabbit anti-Ki67 antibody (Abcam, CAT#ab16667); anti-Bcl-2 (rabbit) antibody (Rockland Immunochemicals, CAT# 200-401-Z43); purified anti-Bax (Biolegend, CAT#633601); cl-caspase-3 (StressMarq Biosciences Inc., CAT# SPC-1319); DeadEnd Fluorometric TUNEL System (Promega, CAT#REF: G3250); Reactive Oxygen Species Assay Kit (Beyotime, CAT#S0033S), Cell Cycle and Apoptosis Analysis Kit (Beyotime, CAT#S0033S and C1052).

Techniques: Activity Assay, Inhibition

IHC assay of the intestinal cancer colo320 xenograft sample from nude mice treated by BF-rTK/GCV ( n = 3). ( A ) The tumor tissue samples were from intestinal cancer colo320 cells treated by PBS/GCV, and BF-rTK/GCV, respectively. The caspase 8 and caspase 3 levels were analyzed with IHC; ( B ) Quantitative analysis of caspase 8 and caspase 3 (* p < 0.05).

Journal: International Journal of Molecular Sciences

Article Title: Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium -Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir

doi: 10.3390/ijms17060891

Figure Lengend Snippet: IHC assay of the intestinal cancer colo320 xenograft sample from nude mice treated by BF-rTK/GCV ( n = 3). ( A ) The tumor tissue samples were from intestinal cancer colo320 cells treated by PBS/GCV, and BF-rTK/GCV, respectively. The caspase 8 and caspase 3 levels were analyzed with IHC; ( B ) Quantitative analysis of caspase 8 and caspase 3 (* p < 0.05).

Article Snippet: The fixed tissues of colo320 intestinal tumor were blocked and incubated with caspase 8 (P18) antibody (BA2143-2, Boster, Wuhan, China), and caspase 3 (P12) antibody (BA3592, Boster), The fixed tissues of MKN-45 gastric cancer tumor colo320 intestinal tumor were blocked and incubated with caspase 8 (P18) antibody (BA2143-2, Boster), TNFR1 antibody (BA4891), TNFR2 antibody (BA1438) and FAS antibody (BA0048-1).

Techniques: